Human LITAF/PIG7/SIMPLE Recombinant Protein (N-GST & C-His) (HP721012)

Name: Human LITAF/PIG7/SIMPLE Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HP721012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP721012
Description	Recombinant Human LITAF/PIG7/SIMPLE Protein, N-GST & C-His (HP721012) expressed in E. coli, spanning Ile92-Leu161. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q99732
Protein length	Ile92-Leu161
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	35.80 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Small integral membrane protein of lysosome/late endosome, LITAF, SIMPLE, p53-induced gene 7 protein, LPS-induced TNF-alpha factor, Lipopolysaccharide-induced tumor necrosis factor-alpha factor, PIG7
Background	Lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF/PIG7) is a ~17 kDa protein. Plays a role in endosomal protein trafficking and in targeting proteins for lysosomal degradation. Plays a role in targeting endocytosed EGFR and ERGG3 for lysosomal degradation, and thereby helps down-regulate downstream signaling cascades. Helps recruit the ESCRT complex components TSG101, HGS and STAM to cytoplasmic membranes. Probably plays a role in regulating protein degradation via its interaction with NEDD4. May also contribute to the regulation of gene expression in the nucleus. 1. Lee, SM. et al. (2012) The Journal of cell biology 199, 799-816. PMID: 23166352 2. Saifi, GM. et al. (2005) Human mutation 25, 372-83. PMID: 15776429 3. Myokai, F. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 4518-23. PMID: 10200294 4. Tang, X. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 5132-7. PMID: 15793005
Note	For research use only.

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SDS-PAGE for Recombinant Human LITAF/PIG7/SIMPLE Protein, N-GST & C-His

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Datasheet

Human LITAF/PIG7/SIMPLE Recombinant Protein (N-GST & C-His).pdf

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Human LITAF/PIG7/SIMPLE Recombinant Protein (N-GST & C-His) (HP721012)

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