Human LRRK2 Recombinant Protein (N-His) (HV789012)

Name: Human LRRK2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV789012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HV789012
Description	Recombinant Human LRRK2 Protein, N-His (HV789012) expressed in E. coli, spanning Phe1479-Pro1729. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q5S007
Protein length	Phe1479-Pro1729
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	42.30 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Dardarin, Leucine-rich repeat serine/threonine-protein kinase 2, PARK8, LRRK2
Background	Leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2/Dardarin) is a ~286 kDa protein. Serine/threonine-protein kinase which phosphorylates a broad range of proteins involved in multiple processes such as neuronal plasticity, innate immunity, autophagy, and vesicle trafficking. Is a key regulator of RAB GTPases by regulating the GTP/GDP exchange and interaction partners of RABs through phosphorylation. Phosphorylates RAB3A, RAB3B, RAB3C, RAB3D, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB29, RAB35, and RAB43. Regulates the RAB3IP-catalyzed GDP/GTP exchange for RAB8A through the phosphorylation of 'Thr-72' on RAB8A. Inhibits the interaction between RAB8A and GDI1 and/or GDI2 by phosphorylating 'Thr-72' on RAB8A. 1. MacLeod, D. et al. (2006) Neuron 52, 587-93. PMID: 17114044 2. Zach, S. et al. (2010) PloS one 5, e13191. PMID: 20949042 3. Angeles, DC. et al. (2011) Human mutation 32, 1390-7. PMID: 21850687 4. Gómez-Suaga, P. et al. (2012) Human molecular genetics 21, 511-25. PMID: 22012985 5. MacLeod, DA. et al. (2013) Neuron 77, 425-39. PMID: 23395371 6. Piccoli, G. et al. (2014) Molecular and cellular biology 34, 2147-61. PMID: 24687852 7. Cho, HJ. et al. (2014) The EMBO journal 33, 2314-31. PMID: 25201882 8. Guerreiro, PS. et al. (2016) Molecular neurobiology 53, 3124-3135. PMID: 26014385 9. Steger, M. et al. (2016) eLife 5. PMID: 26824392 10. Yan, R. et al. (2017) Protein & cell 8, 55-66. PMID: 27830463
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human LRRK2 Protein, N-His
Bioactivity

Detected by Anti-LRRK2/Dardarin Antibody (N241A/34) (Catalog No: HV789053) in indirect ELISA

References

HV789012-SDS-PAGE-1.jpg

Datasheet

Human LRRK2 Recombinant Protein (N-His).pdf

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Formula

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Human LRRK2 Recombinant Protein (N-His) (HV789012)

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