Human LRSAM1/hTAL Recombinant Protein (N-His) (HP559012)

Name: Human LRSAM1/hTAL Recombinant Protein (N-His)
Brand: abinScience
SKU: HP559012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP559012
Description	Recombinant Human LRSAM1/hTAL Protein, N-His (HP559012) expressed in E. coli, spanning Asp32-Glu211. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q6UWE0
Protein length	Asp32-Glu211
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	22.10 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	hTAL, RING-type E3 ubiquitin transferase LRSAM1, Leucine-rich repeat and sterile alpha motif-containing protein 1, TAL, E3 ubiquitin-protein ligase LRSAM1, LRSAM1, Tsg101-associated ligase
Background	E3 ubiquitin-protein ligase LRSAM1 (hTAL) is a ~83 kDa protein. E3 ubiquitin-protein ligase that mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic (EGF receptors) and exocytic (HIV-1 viral proteins) cargos. Bacterial recognition protein that defends the cytoplasm from invasive pathogens. Localizes to several intracellular bacterial pathogens and generates the bacteria-associated ubiquitin signal leading to autophagy-mediated intracellular bacteria degradation (xenophagy). 1. Amit, I. et al. (2004) Genes & development 18, 1737-52. PMID: 15256501 2. Huett, A. et al. (2012) Cell host & microbe 12, 778-90. PMID: 23245322 3. Wang, Z. et al. (2014) Autophagy 10, 2158-70. PMID: 25484098
Note	For research use only.

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SDS-PAGE for Recombinant Human LRSAM1/hTAL Protein, N-His

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Datasheet

Human LRSAM1/hTAL Recombinant Protein (N-His).pdf

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Human LRSAM1/hTAL Recombinant Protein (N-His) (HP559012)

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