

| Catalog No. | HP101022 |
|---|---|
| Description |
Recombinant Human MED15 Protein, N-His (HP101022) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| Expression system | E. coli |
| Accession | Q96RN5 |
| Protein length | Ser676-Ala788 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Homo sapiens (Human) |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | ARC105, Activator-recruited cofactor 105 kDa component, CTG repeat protein 7a, CTG7A, MED15, Mediator complex subunit 15, Mediator of RNA polymerase II transcription subunit 15, PC2 glutamine/Q-rich-associated protein, PCQAP, Positive cofactor 2 glutamine/Q-rich-associated protein, TIG-1, TIG1, TNRC7, TPA-inducible gene 1 protein, Trinucleotide repeat-containing gene 7 protein |
| Background | Mediator of RNA polymerase II transcription subunit 15 is a ~86 kDa protein. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway. |
| Note | For research use only |
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