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Human MED24 Recombinant Protein (N-His) (HC957012)

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Overview
Catalog No.HC957012
Description
Recombinant Human MED24 Protein, N-His (HC957012) expressed in E. coli, spanning Thr278-Thr690. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionO75448
Protein lengthThr278-Thr690
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 48.35 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesARC100, Activator-recruited cofactor 100 kDa component, CRSP complex subunit 4, CRSP4, Cofactor required for Sp1 transcriptional activation subunit 4, DRIP100, KIAA0130, MED24, Mediator complex subunit 24, Mediator of RNA polymerase II transcription subunit 24, THRAP4, TRAP100, Thyroid hormone receptor-associated protein 4, Thyroid hormone receptor-associated protein complex 100 kDa component, Trap100, Vitamin D3 receptor-interacting protein complex 100 kDa component, hTRAP100
Background

Mediator of RNA polymerase II transcription subunit 24 is a ~110 kDa protein. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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