Human METTL14 Recombinant Protein (N-His) (HC797012)

Name: Human METTL14 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC797012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC797012
Description	Recombinant Human METTL14 Protein, N-His (HC797012) expressed in E. coli, spanning Pro147-Ser399. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9HCE5
Protein length	Pro147-Ser399
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	31.51 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	KIAA1627, METTL14, Methyltransferase-like protein 14, N(6)-adenosine-methyltransferase non-catalytic subunit METTL14, hMETTL14
Background	N(6)-adenosine-methyltransferase non-catalytic subunit METTL14 is a ~52 kDa protein. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis. In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing. M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization. 1. Liu, J. et al. (2014) Nature chemical biology 10, 93-5. PMID: 24316715 2. Ping, XL. et al. (2014) Cell research 24, 177-89. PMID: 24407421 3. Liu, N. et al. (2015) Nature 518, 560-4. PMID: 25719671 4. Wang, X. et al. (2016) Nature 534, 575-8. PMID: 27281194 5. Wang, P. et al. (2016) Molecular cell 63, 306-317. PMID: 27373337 6. Schöller, E. et al. (2018) RNA (New York, N.Y.) 24, 499-512. PMID: 29348140 7. Śledź, P. et al. (2016) eLife 5. PMID: 27627798
Note	For research use only

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Human METTL14 Recombinant Protein (N-His).pdf

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Human METTL14 Recombinant Protein (N-His) (HC797012)

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