Human MLLT3 Recombinant Protein (N-His) (HA633012)

Name: Human MLLT3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA633012
Price: 211 USD
Availability: InStock

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Catalog No.	HA633012
Description	Recombinant Human MLLT3 Protein, N-His (HA633012) expressed in E. coli, spanning Met1-Thr148. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P42568
Protein length	Met1-Thr148
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	19.50 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Protein AF-9; ALL1-fused gene from chromosome 9 protein; Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein; YEATS domain-containing protein 3; MLLT3; AF9; YEATS3
Background	Protein AF-9 is a ~63 kDa protein. Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr). Also recognizes and binds histone H3 acetylated and butyrylated at 'Lys-9' (H3K9ac and H3K9bu, respectively), but with lower affinity than crotonylated histone H3. 1. Lin, C. et al. (2010) Molecular cell 37, 429-37. PMID: 20159561 2. He, N. et al. (2010) Molecular cell 38, 428-38. PMID: 20471948 3. Li, Y. et al. (2014) Cell 159, 558-71. PMID: 25417107 4. Li, Y. et al. (2016) Molecular cell 62, 181-193. PMID: 27105114 5. Zhang, Q. et al. (2016) Structure (London, England : 1993) 24, 1606-12. PMID: 27545619 6. Li, X. et al. (2018) Nature chemical biology 14, 1140-1149. PMID: 30374167 7. Klein, BJ. et al. (2018) Nature communications 9, 4574. PMID: 30385749 9. Becht, DC. et al. (2025) Nature structural & molecular biology 32, 709-718. PMID: 39794553
Note	For research use only

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SDS-PAGE for Recombinant Human MLLT3 Protein, N-His

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Datasheet

Human MLLT3 Recombinant Protein (N-His).pdf

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Human MLLT3 Recombinant Protein (N-His) (HA633012)

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