ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
23.70 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
ADP-ribosylation factor-like protein 8B, EC:3.6.5.2, ADP-ribosylation factor-like protein 10C, Novel small G protein indispensable for equal chromosome segregation 1, ARL8B, ARL10C, GIE1
Background
ADP-ribosylation factor-like protein 8B is a ~21 kDa protein. Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of lysosomal positioning which is important for nutrient sensing, natural killer cell-mediated cytotoxicity and antigen presentation. Along with its effectors, orchestrates lysosomal transport and fusion. Localizes specifically to lysosomal membranes and mediates anterograde lysosomal motility by recruiting PLEKHM2, which in turn recruits the motor protein kinesin-1 on lysosomes. Required for lysosomal and cytolytic granule exocytosis.
1. Okai, T. et al. (2004) Journal of cell science 117, 4705-15. PMID: 15331635
2. Hofmann, I. et al. (2006) Journal of cell science 119, 1494-503. PMID: 16537643
3. Bagshaw, RD. et al. (2006) Biochemical and biophysical research communications 344, 1186-91. PMID: 16650381
4. Pu, J. et al. (2015) Developmental cell 33, 176-88. PMID: 25898167
5. Deshar, R. et al. (2016) The FEBS journal 283, 4583-4599. PMID: 27808481
6. Marwaha, R. et al. (2017) The Journal of cell biology 216, 1051-1070. PMID: 28325809
7. Rosa-Ferreira, C. et al. (2011) Developmental cell 21, 1171-8. PMID: 22172677
8. Tuli, A. et al. (2013) Molecular biology of the cell 24, 3721-35. PMID: 24088571
9. Michelet, X. et al. (2018) Journal of immunology (Baltimore, Md. : 1950) 200, 3160-3169. PMID: 29592961
10. Garg, S. et al. (2011) Immunity 35, 182-93. PMID: 21802320
Note
For research use only
Images
SDS-PAGE
SDS-PAGE for Recombinant Human/Mouse/Rat ARL8B Protein, N-His
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