ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
35.06 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
KAT7, HBOa, HBO1, MYST-2, MYST2, Lysine acetyltransferase 7, MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2, Histone acetyltransferase binding to ORC1, Histone acetyltransferase KAT7
Background
Histone acetyltransferase KAT7 is a ~70 kDa protein. Catalytic subunit of histone acetyltransferase HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), thereby regulating various processes, such as gene transcription, protein ubiquitination, immune regulation, stem cell pluripotent and self-renewal maintenance and embryonic development. Some complexes also catalyze acetylation of histone H4 at 'Lys-5', 'Lys-8' and 'Lys-12' (H4K5ac, H4K8ac and H4K12ac, respectively), regulating DNA replication initiation, regulating DNA replication initiation. Specificity of the HBO1 complexes is determined by the scaffold subunit: complexes containing BRPF scaffold (BRPF1, BRD1/BRPF2 or BRPF3) direct KAT7/HBO1 specificity towards H3K14ac, while complexes containing JADE (JADE1, JADE2 and JADE3) scaffold direct KAT7/HBO1 specificity towards histone H4. H3K14ac promotes transcriptional elongation by facilitating the processivity of RNA polymerase II. Acts as a key regulator of hematopoiesis by forming a complex with BRD1/BRPF2, directing KAT7/HBO1 specificity towards H3K14ac and promoting erythroid differentiation.
1. Doyon, Y. et al. (2006) Molecular cell 21, 51-64. PMID: 16387653
2. Mishima, Y. et al. (2011) Blood 118, 2443-53. PMID: 21753189
3. Lalonde, ME. et al. (2013) Genes & development 27, 2009-24. PMID: 24065767
4. Feng, Y. et al. (2016) The EMBO journal 35, 176-92. PMID: 26620551
5. Kueh, AJ. et al. (2020) Molecular and cellular biology 40. PMID: 31767635
6. MacPherson, L. et al. (2020) Nature 577, 266-270. PMID: 31827282
7. Iizuka, M. et al. (1999) The Journal of biological chemistry 274, 23027-34. PMID: 10438470
8. Saksouk, N. et al. (2009) Molecular cell 33, 257-65. PMID: 19187766
9. Miotto, B. et al. (2010) Molecular cell 37, 57-66. PMID: 20129055
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human KAT7 Protein, N-His
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