Human MTA1 Recombinant Protein (N-His) (HA784012)

Name: Human MTA1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA784012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA784012
Description	Recombinant Human MTA1 Protein, N-His (HA784012) expressed in E. coli, spanning Met1-Leu430. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q13330
Protein length	Met1-Leu430
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	51.11 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Metastasis-associated protein MTA1; MTA1
Background	Metastasis-associated protein MTA1 is a ~80 kDa protein. Transcriptional coregulator which can act as both a transcriptional corepressor and coactivator. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. In the NuRD complex, regulates transcription of its targets by modifying the acetylation status of the target chromatin and cofactor accessibility to the target DNA. In conjunction with other components of NuRD, acts as a transcriptional corepressor of BRCA1, ESR1, TFF1 and CDKN1A. Acts as a transcriptional coactivator of BCAS3, and SUMO2, independent of the NuRD complex. 1. Gururaj, AE. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 6670-5. PMID: 16617102 2. Balasenthil, S. et al. (2007) Cancer research 67, 7132-8. PMID: 17671180 3. Molli, PR. et al. (2008) Oncogene 27, 1971-80. PMID: 17922032 4. Cong, L. et al. (2011) The Journal of biological chemistry 286, 43793-43808. PMID: 21965678 5. Kang, HJ. et al. (2014) Cancer research 74, 1484-94. PMID: 24413532 6. Le Guezennec, X. et al. (2006) Molecular and cellular biology 26, 843-51. PMID: 16428440 7. Hoffmeister, H. et al. (2017) Nucleic acids research 45, 10534-10554. PMID: 28977666 8. Li, DQ. et al. (2009) The Journal of biological chemistry 284, 34545-52. PMID: 19837670
Note	For research use only

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SDS-PAGE for Recombinant Human MTA1 Protein, N-His

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Datasheet

Human MTA1 Recombinant Protein (N-His).pdf

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Human MTA1 Recombinant Protein (N-His) (HA784012)

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