Human NAE1 Recombinant Protein (N-His) (HD201012)

Name: Human NAE1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD201012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD201012
Description	Recombinant Human NAE1 Protein, N-His (HD201012) expressed in E. coli, spanning Met1-Leu534. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q13564
Protein length	Met1-Leu534
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	62.41 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	APP-BP1, APPBP1, Amyloid beta precursor protein-binding protein 1, 59 kDa, Amyloid protein-binding protein 1, NAE1, NEDD8-activating enzyme E1 regulatory subunit, Proto-oncogene protein 1
Background	NEDD8-activating enzyme E1 regulatory subunit is a ~60 kDa protein. Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
Note	For research use only

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Datasheet

Human NAE1 Recombinant Protein (N-His).pdf

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Human NAE1 Recombinant Protein (N-His) (HD201012)

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