Human NAT10 Recombinant Protein (N-His) (HC139012)

Name: Human NAT10 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC139012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC139012
Description	Recombinant Human NAT10 Protein, N-His (HC139012) expressed in E. coli, spanning Met1-Asp202. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9H0A0
Protein length	Met1-Asp202
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	25.52 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	18S rRNA cytosine acetyltransferase, ALP, EC:2.3.1.-, KIAA1709, N-acetyltransferase 10, N-acetyltransferase-like protein, NAT10, RNA cytidine acetyltransferase, hALP
Background	RNA cytidine acetyltransferase is a ~115 kDa protein. RNA cytidine acetyltransferase that catalyzes the formation of N(4)-acetylcytidine (ac4C) modification on mRNAs, 18S rRNA and tRNAs. Catalyzes ac4C modification of a broad range of mRNAs, enhancing mRNA stability and translation. mRNA ac4C modification is frequently present within wobble cytidine sites and promotes translation efficiency. Mediates the formation of ac4C at position 1842 in 18S rRNA. May also catalyze the formation of ac4C at position 1337 in 18S rRNA. 1. Ito, S. et al. (2014) The Journal of biological chemistry 289, 35724-30. PMID: 25411247 2. Sharma, S. et al. (2015) Nucleic acids research 43, 2242-58. PMID: 25653167 3. Arango, D. et al. (2018) Cell 175, 1872-1886.e24. PMID: 30449621 4. Arango, D. et al. (2022) Molecular cell 82, 2797-2814.e11. PMID: 35679869
Note	For research use only

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Datasheet

Human NAT10 Recombinant Protein (N-His).pdf

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Human NAT10 Recombinant Protein (N-His) (HC139012)

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