Human NCOA2 Recombinant Protein (N-His) (HA816012)

Name: Human NCOA2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA816012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA816012
Description	Recombinant Human NCOA2 Protein, N-His (HA816012) expressed in E. coli, spanning Val109-Cys380. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q15596
Protein length	Val109-Cys380
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.30 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Nuclear receptor coactivator 2; NCoA-2; Class E basic helix-loop-helix protein 75; bHLHe75; Transcriptional intermediary factor 2; hTIF2; NCOA2; BHLHE75; SRC2; TIF2
Background	Nuclear receptor coactivator 2 is a ~159 kDa protein. Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as a RORA coactivator to specifically modulate G6PC1 expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. 1. Druker, J. et al. (2013) Molecular and cellular biology 33, 2116-27. PMID: 23508108 2. Voegel, JJ. et al. (1996) The EMBO journal 15, 3667-75. PMID: 8670870 3. Voegel, JJ. et al. (1998) The EMBO journal 17, 507-19. PMID: 9430642 4. Musille, PM. et al. (2012) Nature structural & molecular biology 19, 532-S2. PMID: 22504882 5. Musille, PM. et al. (2016) The Journal of biological chemistry 291, 1411-26. PMID: 26553876
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human NCOA2 Protein, N-His

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Datasheet

Human NCOA2 Recombinant Protein (N-His).pdf

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Human NCOA2 Recombinant Protein (N-His) (HA816012)

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