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Human NDUFA8 Recombinant Protein (N-His) (HD089012)

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Overview
Catalog No.HD089012
Description
Recombinant Human NDUFA8 Protein, N-His (HD089012) expressed in E. coli, spanning Val18-Val118. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionP51970
Protein lengthVal18-Val118
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 14.28 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCI-19kD, CI-PGIV, Complex I-19kD, Complex I-PGIV, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, NADH-ubiquinone oxidoreductase 19 kDa subunit, NDUFA8
Background

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 is a ~20 kDa protein. Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

1. Stroud, DA. et al. (2016) Nature 538, 123-126. PMID: 27626371
2. Yatsuka, Y. et al. (2020) Clinical genetics 98, 155-165. PMID: 32385911
3. Tort, F. et al. (2020) Molecular genetics and metabolism 131, 349-357. PMID: 33153867
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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