Human NEK2 Recombinant Protein (N-His) (HW711012)

Name: Human NEK2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HW711012
Price: 211 USD
Availability: InStock

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Notice · Catalog HW711012 has been discontinued.

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Overview

Catalog No.	HW711012
Description	Recombinant Human NEK2 Protein, N-His (HW711012) expressed in E. coli, spanning Lys125-Leu212. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P51955
Protein length	Lys125-Leu212
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	12.34 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	NLK1, NEK2, NimA-like protein kinase 1, HSPK 21, NEK2A, Serine/threonine-protein kinase Nek2, NimA-related protein kinase 2, Never in mitosis A-related kinase 2
Background	Serine/threonine-protein kinase Nek2 is a ~51 kDa protein. Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. 1. Fang, G. et al. (2014) Journal of cell science 127, 1631-9. PMID: 24554434 2. Man, X. et al. (2015) European journal of cell biology 94, 162-72. PMID: 25704143 3. Chen, C. et al. (2015) Journal of cell science 128, 3290-303. PMID: 26220856 4. Xia, Y. et al. (2018) Journal of cell science 131. PMID: 30404835
Note	For research use only.

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SDS-PAGE for Recombinant Human NEK2 Protein, N-His

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Datasheet

Human NEK2 Recombinant Protein (N-His).pdf

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Formula

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Human NEK2 Recombinant Protein (N-His) (HW711012)

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