Human NLRP12 Recombinant Protein (N-His) (HD102012)

Name: Human NLRP12 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD102012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD102012
Description	Recombinant Human NLRP12 Protein, N-His (HD102012) expressed in E. coli, spanning Gln634-Cys1061. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P59046
Protein length	Gln634-Cys1061
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	49.44 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Monarch-1, NACHT, LRR and PYD domains-containing protein 12, NALP12, NLRP12, PYPAF7, PYRIN-containing APAF1-like protein 7, RNO, Regulated by nitric oxide
Background	NACHT, LRR and PYD domains-containing protein 12 is a ~120 kDa protein. Plays an essential role as an potent mitigator of inflammation. Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK activation pathways. Functions as a negative regulator of NOD2 by targeting it to degradation via the proteasome pathway. In turn, promotes bacterial tolerance. Also inhibits the RIGI-mediated immune signaling against RNA viruses by reducing the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked RIGI activation but enhancing the E3 ubiquitin ligase RNF125-mediated 'Lys-48'-linked RIGI degradation. 1. Normand, S. et al. (2018) Nature communications 9, 5338. PMID: 30559449 2. Gerhard, DS. et al. (2004) Genome research 14, 2121-7. PMID: 15489334 3. Arthur, JC. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 179, 6291-6. PMID: 17947705 4. Chen, ST. et al. (2019) Cell host & microbe 25, 602-616.e7. PMID: 30902577
Note	For research use only

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Datasheet

Human NLRP12 Recombinant Protein (N-His).pdf

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Human NLRP12 Recombinant Protein (N-His) (HD102012)

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