Human NLRX1 Recombinant Protein (N-His) (HP387012)

Name: Human NLRX1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP387012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP387012
Description	Recombinant Human NLRX1 Protein, N-His (HP387012) expressed in E. coli, spanning Arg667-Ser975. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q86UT6
Protein length	Arg667-Ser975
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	36.07 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	NLR family member X1, NOD5, Caterpiller protein 11.3, NOD9, Nucleotide-binding oligomerization domain protein 9, NLRX1, Nucleotide-binding oligomerization domain protein 26, Nucleotide-binding oligomerization domain protein 5, NOD26, CLR11.3
Background	NLR family member X1 (NLRX1) is a ~107 kDa protein. Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction. Instead, promotes autophagy by interacting with TUFM and subsequently recruiting the autophagy-related proteins ATG5 and ATG12. Also regulates MAVS-dependent NLRP3 inflammasome activation to attenuate apoptosis. Has no inhibitory function on NF-kappa-B signaling pathway, but enhances NF-kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species. 1. Moore, CB. et al. (2008) Nature 451, 573-7. PMID: 18200010 2. Lei, Y. et al. (2012) Immunity 36, 933-46. PMID: 22749352 3. Li, H. et al. (2016) Molecular immunology 76, 90-7. PMID: 27393910 4. Tattoli, I. et al. (2008) EMBO reports 9, 293-300. PMID: 18219313
Note	For research use only.

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SDS-PAGE for Recombinant Human NLRX1 Protein, N-His

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Datasheet

Human NLRX1 Recombinant Protein (N-His).pdf

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Human NLRX1 Recombinant Protein (N-His) (HP387012)

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