Human NME3 Recombinant Protein (N-His) (HC575012)

Name: Human NME3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC575012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC575012
Description	Recombinant Human NME3 Protein, N-His (HC575012) expressed in E. coli, spanning Met1-Tyr168. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q13232
Protein length	Met1-Tyr168
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	21.05 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	NDPKC, NME3, DR-nm23, Nucleoside diphosphate kinase C, NDP kinase 3, Nucleoside diphosphate kinase 3, NDK 3, nm23-H3
Background	Nucleoside diphosphate kinase C (NME3) is a ~19 kDa protein. Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis. In vitro, can also use other phosphate donors such as UTP and GTP. Independently of its nucleoside diphosphate kinase activity, involved in mitochondrial membrane tethering, a prerequisite for fusion through direct membrane-binding and hexamerization. Involved in DNA repair of both single- and double-stranded breaks by associating with the ribonucleotide reductase (RNR) complex via interaction with the histone acetyltransferase KAT5, facilitating recruitment to DNA damage sites independently of its kinase activity. Inhibits granulocyte differentiation. 1. Erent, M. et al. (2001) European journal of biochemistry 268, 1972-81. PMID: 11277919 2. Chen, CW. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 566-574. PMID: 30587587 3. Amjadi, R. et al. (2024) International journal of molecular sciences 25. PMID: 39337255 4. Su, YA. et al. (2023) The Journal of cell biology 222. PMID: 37584589 5. Tsao, N. et al. (2016) The Biochemical journal 473, 1237-45. PMID: 26945015 6. Venturelli, D. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 7435-9. PMID: 7638209
Note	For research use only.

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SDS-PAGE for Recombinant Human NME3 Protein, N-His

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Datasheet

Human NME3 Recombinant Protein (N-His).pdf

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Formula

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C₁ × V₁ = C₂ × V₂

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Human NME3 Recombinant Protein (N-His) (HC575012)

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