Human NOL9 Recombinant Protein (N-His) (HD295012)

Name: Human NOL9 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD295012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD295012
Description	Recombinant Human NOL9 Protein, N-His (HD295012) expressed in E. coli, spanning Val116-Phe505. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q5SY16
Protein length	Val116-Phe505
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	46.51 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	EC:2.7.1.78, NOL9, Nucleolar protein 9, Polynucleotide 5'-hydroxyl-kinase NOL9
Background	Polynucleotide 5'-hydroxyl-kinase NOL9 is a ~79 kDa protein. Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of single-stranded and double-stranded RNA and DNA substrates. Involved in rRNA processing and its kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form. Required for the efficient pre-rRNA processing of internal transcribed spacer 2 (ITS2). Associates with LAS1L to form an ITS2 pre-rRNA endonuclease-kinase complex and is responsible for the transport of this complex into the nucleolus. 1. Heindl, K. et al. (2010) The EMBO journal 29, 4161-71. PMID: 21063389 2. Gordon, J. et al. (2019) Journal of molecular biology 431, 3771-3786. PMID: 31288032
Note	For research use only

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Human NOL9 Recombinant Protein (N-His).pdf

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Human NOL9 Recombinant Protein (N-His) (HD295012)

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