Human NPRL3 Recombinant Protein (N-His) (HD550012)

Name: Human NPRL3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD550012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD550012
Description	Recombinant Human NPRL3 Protein, N-His (HD550012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	Q12980
Protein length	Met1-Pro569
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	-14 gene protein, Alpha-globin regulatory element-containing gene protein, C16orf35, CGTHBA, GATOR1 complex protein NPRL3, MARE, NPRL3, Nitrogen permease regulator 3-like protein, Protein CGTHBA
Background	GATOR1 complex protein NPRL3 is a ~63 kDa protein. As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway. In response to amino acid depletion, the GATOR1 complex has GTPase activating protein (GAP) activity and strongly increases GTP hydrolysis by RagA/RRAGA (or RagB/RRAGB) within heterodimeric Rag complexes, thereby turning them into their inactive GDP-bound form, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. In the presence of abundant amino acids, the GATOR1 complex is negatively regulated by GATOR2, the other GATOR subcomplex, in this amino acid-sensing branch of the TORC1 pathway. 1. Bar-Peled, L. et al. (2013) Science (New York, N.Y.) 340, 1100-6. PMID: 23723238 2. Shen, K. et al. (2018) Nature 556, 64-69. PMID: 29590090 3. Egri, SB. et al. (2022) Molecular cell 82, 1836-1849.e5. PMID: 35338845
Note	For research use only

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Datasheet

Human NPRL3 Recombinant Protein (N-His).pdf

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Human NPRL3 Recombinant Protein (N-His) (HD550012)

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