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Human NT5C2 Recombinant Protein (N-His) (HD793012)

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Overview
Catalog No.HD793012
Description
Recombinant Human NT5C2 Protein, N-His (HD793012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionP49902
Protein lengthLeu41-Ser474
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCytosolic 5'-nucleotidase II, Cytosolic IMP/GMP-specific 5'-nucleotidase, Cytosolic nucleoside phosphotransferase 5'N, Cytosolic purine 5'-nucleotidase, EC:2.7.1.77, EC:3.1.3.5, EC:3.1.3.99, High Km 5'-nucleotidase, NT5B, NT5C2, NT5CP, PNT5, cN-II
Background

Cytosolic purine 5'-nucleotidase is a ~64 kDa protein. Broad specificity cytosolic 5'-nucleotidase that catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates. In addition, possesses a phosphotransferase activity by which it can transfer a phosphate from a donor nucleoside monophosphate to an acceptor nucleoside, preferably inosine, deoxyinosine and guanosine. Has the highest activities for IMP and GMP followed by dIMP, dGMP and XMP. Could also catalyze the transfer of phosphates from pyrimidine monophosphates but with lower efficiency. Through these activities regulates the purine nucleoside/nucleotide pools within the cell.

1. Spychala, J. et al. (1999) European journal of biochemistry 259, 851-8. PMID: 10092873
2. Mazzon, C. et al. (2003) Biochemical pharmacology 66, 471-9. PMID: 12907246
3. Tozzi, MG. et al. (1991) Archives of biochemistry and biophysics 291, 212-7. PMID: 1659319
4. Allegrini, S. et al. (1997) The Biochemical journal 328 ( Pt 2), 483-7. PMID: 9371705
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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