Human NTHL1 Recombinant Protein (N-His) (HD084012)

Name: Human NTHL1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD084012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD084012
Description	Recombinant Human NTHL1 Protein, N-His (HD084012) expressed in E. coli, spanning Gln90-Leu312. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P78549
Protein length	Gln90-Leu312
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	27.01 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase, DNA glycosylase/AP lyase, EC:3.2.2.-, EC:4.2.99.18, Endonuclease III-like protein 1, NTH1, NTHL1, OCTS3, hNTH1
Background	Endonuclease III-like protein 1 is a ~33 kDa protein. Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Also has 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. 1. Williams, SC. et al. (2018) Molecular and cellular biology 38. PMID: 29610152 2. Bessho, T. (1999) Nucleic acids research 27, 979-83. PMID: 9927729
Note	For research use only

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Datasheet

Human NTHL1 Recombinant Protein (N-His).pdf

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Human NTHL1 Recombinant Protein (N-His) (HD084012)

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