Human NUF2 Recombinant Protein (N-His) (HC721012)

Name: Human NUF2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC721012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC721012
Description	Recombinant Human NUF2 Protein, N-His (HC721012) expressed in E. coli, spanning Met1-Ser171. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9BZD4
Protein length	Met1-Ser171
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	22.24 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CDCA1, Cell division cycle-associated protein 1, Kinetochore protein Nuf2, NUF2, NUF2R, hNuf2, hNuf2R, hsNuf2
Background	Kinetochore protein Nuf2 is a ~54 kDa protein. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. 1. DeLuca, JG. et al. (2002) The Journal of cell biology 159, 549-55. PMID: 12438418 2. DeLuca, JG. et al. (2003) Current biology : CB 13, 2103-9. PMID: 14654001 3. Joseph, J. et al. (2004) Current biology : CB 14, 611-7. PMID: 15062103 4. Stucke, VM. et al. (2004) Chromosoma 113, 1-15. PMID: 15235793 5. Meraldi, P. et al. (2004) Developmental cell 7, 45-60. PMID: 15239953 6. DeLuca, JG. et al. (2005) Molecular biology of the cell 16, 519-31. PMID: 15548592 7. Liu, D. et al. (2007) The Journal of biological chemistry 282, 21415-24. PMID: 17535814 8. Schmidt, JC. et al. (2012) Developmental cell 23, 968-80. PMID: 23085020
Note	For research use only

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Datasheet

Human NUF2 Recombinant Protein (N-His).pdf

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Human NUF2 Recombinant Protein (N-His) (HC721012)

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