Human ORAI3 Recombinant Protein (N-His-SUMO) (HC722012)

Name: Human ORAI3 Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: HC722012
Price: 211 USD
Availability: InStock

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Catalog No.	HC722012
Description	Recombinant Human ORAI3 Protein, N-His-SUMO (HC722012) expressed in E. coli, spanning Gly178-Gln243. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9BRQ5
Protein length	Gly178-Gln243
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	18.68 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ORAI3, Protein orai-3, TMEM142C, Transmembrane protein 142C
Background	Protein orai-3 is a ~31 kDa protein. Pore-forming subunit of two major inward rectifying Ca(2+) channels at the plasma membrane: Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels. Assembles with ORAI1 and ORAI2 to form hexameric CRAC channels that mediate Ca(2+) influx upon depletion of endoplasmic reticulum Ca(2+) store and channel activation by Ca(2+) sensor STIM1, a process known as store-operated Ca(2+) entry (SOCE). Various pore subunit combinations may account for distinct CRAC channel spatiotemporal and cell-type specific dynamics. ORAI1 mainly contributes to the generation of Ca(2+) plateaus involved in sustained Ca(2+) entry and is dispensable for cytosolic Ca(2+) oscillations, whereas ORAI2 and ORAI3 generate oscillatory patterns. CRAC channels assemble in Ca(2+) signaling microdomains where Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT transcription factors recruited to ORAI1 via AKAP5. 1. Mercer, JC. et al. (2006) The Journal of biological chemistry 281, 24979-90. PMID: 16807233 2. Lis, A. et al. (2007) Current biology : CB 17, 794-800. PMID: 17442569 3. Yuan, JP. et al. (2009) Nature cell biology 11, 337-43. PMID: 19182790 4. Mignen, O. et al. (2009) The Journal of physiology 587, 4181-97. PMID: 19622606 5. Lee, KP. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 14687-92. PMID: 19706554 6. Bogeski, I. et al. (2010) Science signaling 3, ra24. PMID: 20354224 7. Yoast, RE. et al. (2020) Nature communications 11, 2444. PMID: 32415068
Note	For research use only

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Datasheet

Human ORAI3 Recombinant Protein (N-His-SUMO).pdf

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Human ORAI3 Recombinant Protein (N-His-SUMO) (HC722012)

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