Human OSBP Recombinant Protein (N-His) (HC195012)

Name: Human OSBP Recombinant Protein (N-His)
Brand: abinScience
SKU: HC195012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC195012
Description	Recombinant Human OSBP Protein, N-His (HC195012) expressed in E. coli, spanning Ser416-Lys654. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P22059
Protein length	Ser416-Lys654
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	29.63 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	OSBP, OSBP1, Oxysterol-binding protein 1
Background	Oxysterol-binding protein 1 is a ~89 kDa protein. Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol (cholesterol) with phosphatidylinositol 4-phosphate (PI4P, 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)), delivering sterol to the Golgi in exchange for PI4P, which is subsequently degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing the stability of the relatively short lived ABCA1 protein (an important point of control for cholesterol efflux activity). 1. Mesmin, B. et al. (2013) Cell 155, 830-43. PMID: 24209621 2. Mesmin, B. et al. (2017) The EMBO journal 36, 3156-3174. PMID: 28978670 3. Wang, PY. et al. (2005) Science (New York, N.Y.) 307, 1472-6. PMID: 15746430 4. Suchanek, M. et al. (2007) The Biochemical journal 405, 473-80. PMID: 17428193 5. Bowden, K. et al. (2008) The Journal of biological chemistry 283, 18210-7. PMID: 18450749
Note	For research use only

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Datasheet

Human OSBP Recombinant Protein (N-His).pdf

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Human OSBP Recombinant Protein (N-His) (HC195012)

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