Human PARG Recombinant Protein (N-His) (HV273012)

Name: Human PARG Recombinant Protein (N-His)
Brand: abinScience
SKU: HV273012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HV273012
Description	Recombinant Human PARG Protein, N-His (HV273012) expressed in E. coli, spanning Ser448-Thr976. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q86W56
Protein length	Ser448-Thr976
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Remove endotoxin and sterilization is required for cell assay.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	63.36 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	PARG, Poly(ADP-ribose) glycohydrolase
Background	Poly(ADP-ribose) glycohydrolase (PARG) is a ~111 kDa protein. Poly(ADP-ribose) glycohydrolase that degrades poly(ADP-ribose) by hydrolyzing the ribose-ribose bonds present in poly(ADP-ribose). Mainly acts as an exo-glycohydrolase but can act as an endo-glycohydrolase at low efficiency, releasing poly(ADP-ribose) of different length as well as ADP-ribose monomers. It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated. Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by PARG. Required to prevent detrimental accumulation of poly(ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress. 1. Putt, KS. et al. (2004) Analytical biochemistry 333, 256-64. PMID: 15450800 2. Slade, D. et al. (2011) Nature 477, 616-20. PMID: 21892188 3. Le May, N. et al. (2012) Molecular cell 48, 785-98. PMID: 23102699 4. Rosenthal, F. et al. (2013) Nature structural & molecular biology 20, 502-7. PMID: 23474714 5. Bonfiglio, JJ. et al. (2020) Cell 183, 1086-1102.e23. PMID: 33186521 6. Prokhorova, E. et al. (2021) Molecular cell 81, 2640-2655.e8. PMID: 34019811 7. Rack, JGM. et al. (2021) Nature communications 12, 4581. PMID: 34321462 8. Sharifi, R. et al. (2013) The EMBO journal 32, 1225-37. PMID: 23481255 9. Fontana, P. et al. (2023) Nature communications 14, 3200. PMID: 37268618
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Human PARG Recombinant Protein (N-His)

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Datasheet

Human PARG Recombinant Protein (N-His).pdf

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Formula

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Human PARG Recombinant Protein (N-His) (HV273012)

Overview

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References

Datasheet

SDS