ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
22.05 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Parkinson disease protein 7, Oncogene DJ1, Parkinsonism-associated deglycase, DJ-1, PARK7, Maillard deglycase, Protein/nucleic acid deglycase DJ-1, Protein DJ-1
Background
Parkinson disease protein 7 (PARK7) is a ~19 kDa protein. Multifunctional protein with controversial molecular function which plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease. It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway. Has been described as a protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. But this function is rebuted by other works. As a protein deglycase, repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively.
1. Honbou, K. et al. (2003) The Journal of biological chemistry 278, 31380-4. PMID: 12796482
2. Clements, CM. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 15091-6. PMID: 17015834
3. Junn, E. et al. (2009) Journal of neuroscience research 87, 123-9. PMID: 18711745
4. Xiong, H. et al. (2009) The Journal of clinical investigation 119, 650-60. PMID: 19229105
5. Chen, J. et al. (2010) Human molecular genetics 19, 2395-408. PMID: 20304780
6. Richarme, G. et al. (2015) The Journal of biological chemistry 290, 1885-97. PMID: 25416785
7. Advedissian, T. et al. (2016) Biochemical and biophysical research communications 473, 87-91. PMID: 26995087
8. Matsuda, N. et al. (2017) Scientific reports 7, 12816. PMID: 28993701
9. Niki, T. et al. (2003) Molecular cancer research : MCR 1, 247-61. PMID: 12612053
10. Taira, T. et al. (2004) EMBO reports 5, 213-8. PMID: 14749723
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human PARK7 Protein, N-His
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