Human PDE10A Recombinant Protein (N-His) (HV361012)

Name: Human PDE10A Recombinant Protein (N-His)
Brand: abinScience
SKU: HV361012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HV361012
Description	Recombinant Human PDE10A Protein, N-His (HV361012) expressed in E. coli, spanning Asn508-Asp779. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9Y233
Protein length	Asn508-Asp779
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.29 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	cAMP and cAMP-inhibited cGMP 3', 5'-cyclic phosphodiesterase 10A, PDE10A
Background	cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A (PDE10A) is a ~114 kDa protein. Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. May play a critical role in regulating cAMP and cGMP levels in the striatum, a region of the brain that contributes to the control of movement and cognition. 1. Fujishige, K. et al. (1999) The Journal of biological chemistry 274, 18438-45. PMID: 10373451 2. Loughney, K. et al. (1999) Gene 234, 109-17. PMID: 10393245 3. Gross-Langenhoff, M. et al. (2006) The Journal of biological chemistry 281, 2841-6. PMID: 16330539 4. Wang, H. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 5782-7. PMID: 17389385 5. Mencacci, NE. et al. (2016) American journal of human genetics 98, 763-71. PMID: 27058447
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human PDE10A Protein, N-His

References

HV361012-SDS-PAGE-1.jpg

Datasheet

Human PDE10A Recombinant Protein (N-His).pdf

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Formula

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Human PDE10A Recombinant Protein (N-His) (HV361012)

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