Human PEX12 Recombinant Protein (N-GST & C-His) (HT031012)

Name: Human PEX12 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HT031012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT031012
Description	Recombinant Human PEX12 Protein, N-GST & C-His (HT031012) expressed in E. coli, spanning Ala278-Asn359. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O00623
Protein length	Ala278-Asn359
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	37.47 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Peroxisome assembly protein 12, Peroxin-12, Peroxisome assembly factor 3, PAF-3, PEX12, PAF3
Background	Peroxisome assembly protein 12 (PEX12) is a ~40 kDa protein. Component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX12 also regulates PEX5 recycling by activating the E3 ubiquitin-protein ligase activity of PEX10. When PEX5 recycling is compromised, PEX12 stimulates PEX10-mediated polyubiquitination of PEX5, leading to its subsequent degradation. 1. Okumoto, K. et al. (2014) The Journal of biological chemistry 289, 14089-108. PMID: 24662292 2. Okumoto, K. et al. (1997) Nature genetics 17, 265-6. PMID: 9354782 3. Okumoto, K. et al. (1998) Molecular and cellular biology 18, 4324-36. PMID: 9632816
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human PEX12 Protein, N-GST & C-His

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Datasheet

Human PEX12 Recombinant Protein (N-GST & C-His).pdf

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Human PEX12 Recombinant Protein (N-GST & C-His) (HT031012)

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