Human PGAM5 Recombinant Protein (N-His) (HB500012)

Name: Human PGAM5 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB500012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB500012
Description	Recombinant Human PGAM5 Protein, N-His (HB500012) expressed in E. coli, spanning Ala94-Ser289. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q96HS1
Protein length	Ala94-Ser289
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	24.50 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Serine/threonine-protein phosphatase PGAM5, mitochondrial; 3.1.3.16; Bcl-XL-binding protein v68; Phosphoglycerate mutase family member 5; PGAM5
Background	Serine/threonine-protein phosphatase PGAM5, mitochondrial is a ~32 kDa protein. Mitochondrial serine/threonine phosphatase that dephosphorylates various substrates and thus plays a role in different biological processes including cellular senescence or mitophagy. Modulates cellular senescence by regulating mitochondrial dynamics. Mechanistically, participates in mitochondrial fission through dephosphorylating DNM1L/DRP1. Additionally, dephosphorylates MFN2 in a stress-sensitive manner and consequently protects it from ubiquitination and degradation to promote mitochondrial network formation. Regulates mitophagy independent of PARKIN by interacting with and dephosphorylating FUNDC1, which interacts with LC3. 1. Chen, G. et al. (2014) Molecular cell 54, 362-77. PMID: 24746696 2. Yu, B. et al. (2020) Nature communications 11, 2549. PMID: 32439975 3. Nag, S. et al. (2023) Cell reports 42, 112895. PMID: 37498743 4. Lo, SC. et al. (2008) Experimental cell research 314, 1789-803. PMID: 18387606 5. Wang, Z. et al. (2012) Cell 148, 228-43. PMID: 22265414
Note	For research use only

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SDS-PAGE for Recombinant Human PGAM5 Protein, N-His

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Datasheet

Human PGAM5 Recombinant Protein (N-His).pdf

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Human PGAM5 Recombinant Protein (N-His) (HB500012)

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