Human PHF1 Recombinant Protein (N-His) (HC900012)

Name: Human PHF1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC900012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC900012
Description	Recombinant Human PHF1 Protein, N-His (HC900012) expressed in E. coli, spanning Leu31-Arg334. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O43189
Protein length	Leu31-Arg334
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	36.90 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	PCL1, PHD finger protein 1, PHF1, Polycomb-like protein 1, Protein PHF1, hPCl1, hPHF1
Background	PHD finger protein 1 is a ~62 kDa protein. Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci. According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2. 1. Sarma, K. et al. (2008) Molecular and cellular biology 28, 2718-31. PMID: 18285464 2. Cai, L. et al. (2013) Molecular cell 49, 571-82. PMID: 23273982 3. Musselman, CA. et al. (2012) Nature structural & molecular biology 19, 1266-72. PMID: 23142980
Note	For research use only

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Datasheet

Human PHF1 Recombinant Protein (N-His).pdf

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Human PHF1 Recombinant Protein (N-His) (HC900012)

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