Human PHF19 Recombinant Protein (N-His) (HC221012)

Name: Human PHF19 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC221012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC221012
Description	Recombinant Human PHF19 Protein, N-His (HC221012) expressed in E. coli, spanning Asn22-Lys350. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q5T6S3
Protein length	Asn22-Lys350
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	40.20 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	PCL3, PHD finger protein 19, PHF19, Polycomb-like protein 3, hPCL3
Background	PHD finger protein 19 is a ~65 kDa protein. Polycomb group (PcG) protein that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex, thus enhancing PRC2 H3K27me3 methylation activity. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds histone H3 dimethylated at 'Lys-36' (H3K36me2). Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter. 1. Wang, S. et al. (2004) Gene 343, 69-78. PMID: 15563832 2. Daub, H. et al. (2008) Molecular cell 31, 438-48. PMID: 18691976 3. Ballaré, C. et al. (2012) Nature structural & molecular biology 19, 1257-65. PMID: 23104054 4. Brien, GL. et al. (2012) Nature structural & molecular biology 19, 1273-81. PMID: 23160351 5. Qin, S. et al. (2013) Biochemical and biophysical research communications 430, 547-53. PMID: 23228662 6. Cai, L. et al. (2013) Molecular cell 49, 571-82. PMID: 23273982 7. Chen, S. et al. (2018) Molecular cell 69, 840-852.e5. PMID: 29499137 8. Chen, S. et al. (2020) Molecular cell 77, 1265-1278.e7. PMID: 31959557
Note	For research use only

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Datasheet

Human PHF19 Recombinant Protein (N-His).pdf

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Human PHF19 Recombinant Protein (N-His) (HC221012)

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