ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
16.96 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
C14orf9, EC:3.1.3.78, PIP4P1, PtdIns-4,5-P2 4-Ptase I, TMEM55B, Transmembrane protein 55B, Type 1 PtdIns-4,5-P2 4-Ptase, Type 1 phosphatidylinositol 4,5-bisphosphate 4-phosphatase
Background
Type 1 phosphatidylinositol 4,5-bisphosphate 4-phosphatase is a ~29 kDa protein. Catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2) to phosphatidylinositol-4-phosphate (PtdIns-4-P). Does not hydrolyze phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-bisphosphate, inositol 3,5-bisphosphate, inositol 3,4-bisphosphate, phosphatidylinositol 5-monophosphate, phosphatidylinositol 4-monophosphate and phosphatidylinositol 3-monophosphate. Regulates lysosomal positioning by recruiting JIP4 to lysosomal membranes, thus inducing retrograde transport of lysosomes along microtubules. Contributes to assembly of the V-ATPase complex in lipid rafts of the lysosomal membrane and to subsequent amino acid-dependent activation of mTORC1. May play a role in the regulation of cellular cholesterol metabolism.
1. Ungewickell, A. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 18854-9. PMID: 16365287
2. Willett, R. et al. (2017) Nature communications 8, 1580. PMID: 29146937
3. Hashimoto, Y. et al. (2018) Genes to cells : devoted to molecular & cellular mechanisms 23, 418-434. PMID: 29644770
4. Medina, MW. et al. (2014) Arteriosclerosis, thrombosis, and vascular biology 34, 1917-23. PMID: 25035345
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