Human PLOD1 Recombinant Protein (N-GST) (HX220012)

Name: Human PLOD1 Recombinant Protein (N-GST)
Brand: abinScience
SKU: HX220012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX220012
Description	Recombinant Human PLOD1 Protein, N-GST (HX220012) expressed in E. coli, spanning Gln636-Pro727. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q02809
Protein length	Gln636-Pro727
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	37.33 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1, Lysyl hydroxylase 1, LH1, PLOD, PLOD1, LLH
Background	Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1) is a ~83 kDa protein. Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils. Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (Probable). 1. Yeowell, HN. et al. (2000) Matrix biology : journal of the International Society for Matrix Biology 19, 37-46. PMID: 10686424 2. Walker, LC. et al. (2005) The Journal of investigative dermatology 124, 914-8. PMID: 15854030 3. Pirskanen, A. et al. (1996) The Journal of biological chemistry 271, 9398-402. PMID: 8621606
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human PLOD1 Protein, N-GST

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Datasheet

Human PLOD1 Recombinant Protein (N-GST).pdf

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Formula

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Human PLOD1 Recombinant Protein (N-GST) (HX220012)

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