Human POLR2D Recombinant Protein (N-His) (HC895012)

Name: Human POLR2D Recombinant Protein (N-His)
Brand: abinScience
SKU: HC895012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC895012
Description	Recombinant Human POLR2D Protein, N-His (HC895012) expressed in E. coli, spanning Met1-Tyr142. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O15514
Protein length	Met1-Tyr142
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	18.47 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DNA-directed RNA polymerase II subunit D, DNA-directed RNA polymerase II subunit RPB4, POLR2D, RNA polymerase II 16 kDa subunit, RNA polymerase II subunit B4, RPB16
Background	DNA-directed RNA polymerase II subunit RPB4 is a ~16 kDa protein. Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. POLR2D/RPB4 is part of a subcomplex with POLR2G/RPB7 that binds to a pocket formed by POLR2A/RPB1, POLR2B/RPB2 and POLR2F/RPABC2 at the base of the clamp element. The POLR2D/RPB4-POLR2G/RPB7 subcomplex seems to lock the clamp via POLR2G/RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft.
Note	For research use only

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Datasheet

Human POLR2D Recombinant Protein (N-His).pdf

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Human POLR2D Recombinant Protein (N-His) (HC895012)

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