Human PRKAG2 Recombinant Protein (N-His) (HC755012)

Name: Human PRKAG2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC755012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC755012
Description	Recombinant Human PRKAG2 Protein, N-His (HC755012) expressed in E. coli, spanning Glu251-Glu569. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9UGJ0
Protein length	Glu251-Glu569
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	38.67 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	5'-AMP-activated protein kinase subunit gamma-2, AMPK gamma2, AMPK subunit gamma-2, H91620p, PRKAG2
Background	5'-AMP-activated protein kinase subunit gamma-2 is a ~63 kDa protein. AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. 1. Scott, JW. et al. (2004) The Journal of clinical investigation 113, 274-84. PMID: 14722619 2. Bullen, JW. et al. (2014) The Journal of biological chemistry 289, 10592-10606. PMID: 24563466
Note	For research use only

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Datasheet

Human PRKAG2 Recombinant Protein (N-His).pdf

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Human PRKAG2 Recombinant Protein (N-His) (HC755012)

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