Human PRKN Recombinant Protein (N-His) (HT315012)

Name: Human PRKN Recombinant Protein (N-His)
Brand: abinScience
SKU: HT315012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT315012
Description	Recombinant Human PRKN Protein, N-His (HT315012) expressed in E. coli, spanning Ile229-Val465. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O60260
Protein length	Ile229-Val465
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	28.99 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Parkin, PARK2, Parkinson juvenile disease protein 2, E3 ubiquitin-protein ligase parkin, Parkin RBR E3 ubiquitin-protein ligase, PRKN, Parkinson disease protein 2
Background	E3 ubiquitin-protein ligase parkin (PRKN) is a ~51 kDa protein. Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Substrates include SYT11 and VDAC1. Other substrates are BCL2, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPTIN5, TOMM20, USP30, ZNF746, MIRO1 and AIMP2. Mediates monoubiquitination as well as 'Lys-6', 'Lys-11', 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation. 1. Shimura, H. et al. (2000) Nature genetics 25, 302-5. PMID: 10888878 2. Imai, Y. et al. (2000) The Journal of biological chemistry 275, 35661-4. PMID: 10973942 3. Shimura, H. et al. (2001) Science (New York, N.Y.) 293, 263-9. PMID: 11431533 4. Imai, Y. et al. (2002) Molecular cell 10, 55-67. PMID: 12150907 5. Staropoli, JF. et al. (2003) Neuron 37, 735-49. PMID: 12628165 6. Chung, KK. et al. (2004) Science (New York, N.Y.) 304, 1328-31. PMID: 15105460 7. Ko, HS. et al. (2005) The Journal of neuroscience : the official journal of the Society for Neuroscience 25, 7968-78. PMID: 16135753 8. Shin, JH. et al. (2011) Cell 144, 689-702. PMID: 21376232 9. Wenzel, DM. et al. (2011) Nature 474, 105-8. PMID: 21532592 10. Liu, S. et al. (2012) PLoS genetics 8, e1002537. PMID: 22396657
Note	For research use only.

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SDS-PAGE for Recombinant Human PRKN Protein, N-His

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HT315012-SDS-PAGE-1.jpg

Datasheet

Human PRKN Recombinant Protein (N-His).pdf

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Human PRKN Recombinant Protein (N-His) (HT315012)

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