ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
20.82 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
PTH/PTHr receptor, Parathyroid hormone 1 receptor, PTHR1, PTHR, PTH1 receptor, PTH/PTHrP type I receptor, PTH1R, Parathyroid hormone/parathyroid hormone-related peptide receptor
Background
Parathyroid hormone/parathyroid hormone-related peptide receptor (PTH1R) is a ~66 kDa protein. G-protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP). PTH1R is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity. PTHLH dissociates from PTH1R more rapidly than PTH; as consequence, the cAMP response induced by PTHLH decays faster than the response induced by PTH.
1. Grauschopf, U. et al. (2000) Biochemistry 39, 8878-87. PMID: 10913300
2. Pioszak, AA. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 5034-9. PMID: 18375760
3. Pioszak, AA. et al. (2009) The Journal of biological chemistry 284, 28382-28391. PMID: 19674967
4. Shimomura-Kuroki, J. et al. (2017) Odontology 105, 150-154. PMID: 27160269
5. Zhao, LH. et al. (2019) Science (New York, N.Y.) 364, 148-153. PMID: 30975883
6. Kobayashi, K. et al. (2022) Molecular cell 82, 3468-3483.e5. PMID: 35932760
7. Schneider, H. et al. (1993) European journal of pharmacology 246, 149-55. PMID: 8397094
8. Pioszak, AA. et al. (2010) The Journal of biological chemistry 285, 12435-44. PMID: 20172855
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human PTH1R Protein, N-His
Bioactivity
Detected by SAA0797 (Catalog No: HX947013) in indirect ELISA.
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