Human RAD23B/HR23B Recombinant Protein (N-His) (HX150011)

Name: Human RAD23B/HR23B Recombinant Protein (N-His)
Brand: abinScience
SKU: HX150011
Price: 298 USD
Availability: InStock

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Overview

Catalog No.	HX150011
Description	Recombinant Human RAD23B/HR23B Protein, N-His (HX150011) expressed in Mammalian Cells, spanning Gln2-Asp409. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian cells
Accession	P54727
Protein length	Gln2-Asp409
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	44.28 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	UV excision repair protein RAD23 homolog B, p58, HR23B, RAD23B, XP-C repair-complementing complex 58 kDa protein, hHR23B
Background	Lysine-specific demethylase RAD23B (HR23B) is a ~43 kDa protein. Multifunctional protein that participates in histone H4K20 demethylation, DNA repair, ubiquitin-dependent protein degradation and transcriptional regulation. Specifically demethylates mono-, di- and trimethylated 'Lys-20' of histone H4 (H4K20me1, H4K20me2, H4K20me3, respectively) into unmethylated forms. Activates the transcription of coding genes by demethylating H4K20me1 and the transcription of repetitive elements by demethylating H4K20me3. Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. 1. Hiyama, H. et al. (1999) The Journal of biological chemistry 274, 28019-25. PMID: 10488153 2. Cao, X. et al. (2020) Cell reports 30, 4152-4164.e6. PMID: 32209475 3. Sugasawa, K. et al. (1997) Molecular and cellular biology 17, 6924-31. PMID: 9372924 4. Li, X. et al. (2009) The Biochemical journal 421, 397-404. PMID: 19435460 5. Katiyar, S. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 13774-9. PMID: 15358861 6. Batty, D. et al. (2000) Journal of molecular biology 300, 275-90. PMID: 10873465 7. Ng, JM. et al. (2003) Genes & development 17, 1630-45. PMID: 12815074
Note	For research use only.

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SDS-PAGE for Recombinant Human RAD23B/HR23B Protein, N-His

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Datasheet

Human RAD23B/HR23B Recombinant Protein (N-His).pdf

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Human RAD23B/HR23B Recombinant Protein (N-His) (HX150011)

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