Human RBM24 Recombinant Protein (N-His-SUMO & C-Strep) (HP302012)

Name: Human RBM24 Recombinant Protein (N-His-SUMO & C-Strep)
Brand: abinScience
SKU: HP302012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP302012
Description	Recombinant Human RBM24 Protein, N-His-SUMO & C-Strep (HP302012) expressed in E. coli, spanning Thr11-Lys91. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9BX46
Protein length	Thr11-Lys91
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	22.51 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	RNA-binding region-containing protein 6, RNA-binding protein 24, RNA-binding motif protein 24, RBM24, RNPC6
Background	RNA-binding protein 24 (RBM24) is a ~24 kDa protein. Multifunctional RNA-binding protein involved in the regulation of pre-mRNA splicing, mRNA stability and mRNA translation important for cell fate decision and differentiation. Plays a major role in pre-mRNA alternative splicing regulation. Mediates preferentially muscle-specific exon inclusion in numerous mRNAs important for striated cardiac and skeletal muscle cell differentiation. Binds to intronic splicing enhancer (ISE) composed of stretches of GU-rich motifs localized in flanking intron of exon that will be included by alternative splicing. Involved in embryonic stem cell (ESC) transition to cardiac cell differentiation by promoting pre-mRNA alternative splicing events of several pluripotency and/or differentiation genes. 1. Jin, D. et al. (2010) Genes to cells : devoted to molecular & cellular mechanisms 15, 1158-67. PMID: 20977548 2. Xu, E. et al. (2014) Molecular cancer research : MCR 12, 359-69. PMID: 24375645 3. Lin, Y. et al. (2018) The international journal of biochemistry & cell biology 94, 10-21. PMID: 29104163 4. Zhang, M. et al. (2018) Cell death and differentiation 25, 1118-1130. PMID: 29358667 5. Zhang, T. et al. (2016) Stem cells (Dayton, Ohio) 34, 1776-89. PMID: 26990106 6. Jiang, Y. et al. (2014) The Journal of biological chemistry 289, 3164-75. PMID: 24356969
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human RBM24 Protein, N-His-SUMO & C-Strep

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Datasheet

Human RBM24 Recombinant Protein (N-His-SUMO & C-Strep).pdf

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Human RBM24 Recombinant Protein (N-His-SUMO & C-Strep) (HP302012)

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