Human RFTN1 Recombinant Protein (N-His) (HC659012)

Name: Human RFTN1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC659012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC659012
Description	Recombinant Human RFTN1 Protein, N-His (HC659012) expressed in E. coli, spanning Ser288-Leu520. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q14699
Protein length	Ser288-Leu520
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	28.46 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	KIAA0084, Raft-linking protein, Raftlin, RFTN1, Cell migration-inducing gene 2 protein
Background	Raftlin (RFTN1) is a ~63 kDa protein. Involved in protein trafficking via association with clathrin and AP2 complex. Upon bacterial lipopolysaccharide stimulation, mediates internalization of TLR4 to endosomes in dendritic cells and macrophages; and internalization of poly(I:C) to TLR3-positive endosomes in myeloid dendritic cells and epithelial cells; resulting in activation of TICAM1-mediated signaling and subsequent IFNB1 production. Involved in T-cell antigen receptor-mediated signaling by regulating tyrosine kinase LCK localization, T-cell dependent antibody production and cytokine secretion. May regulate B-cell antigen receptor-mediated signaling. May play a pivotal role in the formation and/or maintenance of lipid rafts. 1. Watanabe, A. et al. (2011) The Journal of biological chemistry 286, 10702-11. PMID: 21266579 2. Tatematsu, M. et al. (2016) Journal of immunology (Baltimore, Md. : 1950) 196, 3865-76. PMID: 27022195 3. Saeki, K. et al. (2003) The EMBO journal 22, 3015-26. PMID: 12805216
Note	For research use only.

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SDS-PAGE for Recombinant Human RFTN1 Protein, N-His

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Datasheet

Human RFTN1 Recombinant Protein (N-His).pdf

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Human RFTN1 Recombinant Protein (N-His) (HC659012)

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