Human RNF19B Recombinant Protein (N-His) (HB475012)

Name: Human RNF19B Recombinant Protein (N-His)
Brand: abinScience
SKU: HB475012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB475012
Description	Recombinant Human RNF19B Protein, N-His (HB475012) expressed in E. coli, spanning Ala114-Ser323. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q6ZMZ0
Protein length	Ala114-Ser323
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	26.33 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	E3 ubiquitin-protein ligase RNF19B; 2.3.2.31; IBR domain-containing protein 3; Natural killer lytic-associated molecule; RING finger protein 19B; RNF19B; IBRDC3; NKLAM
Background	E3 ubiquitin-protein ligase RNF19B is a ~77 kDa protein. E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as UCKL1. Involved in the cytolytic activity of natural killer cells and cytotoxic T-cells. Protects against staurosporin-induced cell death. 1. Fortier, JM. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 6454-63. PMID: 16709802 2. Kaneko, M. et al. (2016) Scientific reports 6, 30955. PMID: 27485036 3. Kozlowski, M. et al. (1999) Journal of immunology (Baltimore, Md. : 1950) 163, 1775-85. PMID: 10438909
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human RNF19B Protein, N-His

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Datasheet

Human RNF19B Recombinant Protein (N-His).pdf

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Human RNF19B Recombinant Protein (N-His) (HB475012)

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