Human rotavirus A Outer capsid protein VP4 Recombinant Protein (N-His) (VK676022)

Name: Human rotavirus A Outer capsid protein VP4 Recombinant Protein (N-His)
Brand: abinScience
SKU: VK676022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK676022
Description	Recombinant Human rotavirus A Outer capsid protein VP4 Protein, N-His (VK676022) expressed in E. coli, spanning Ala248-Tyr480. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P12976
Protein length	Ala248-Tyr480
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Rotavirus A
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	28.64 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Outer capsid protein VP4, Hemagglutinin, Outer capsid protein VP8, Outer capsid protein VP5
Background	Outer capsid protein VP4 is a ~86 kDa protein. Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. It is subsequently lost, together with VP7, following virus entry into the host cell. Following entry into the host cell, low intracellular or intravesicular Ca(2+) concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion. This step is probably necessary for the membrane-disrupting entry step and the release of VP4, which is locked onto the virion by VP7.
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human rotavirus A Outer capsid protein VP4 Protein, N-His

References

Datasheet

Human rotavirus A Outer capsid protein VP4 Recombinant Protein (N-His).pdf

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Human rotavirus A Outer capsid protein VP4 Recombinant Protein (N-His) (VK676022)

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References

Datasheet

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