ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
32.06 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
p90-RSK 1, Ribosomal S6 kinase 1, RSK1, p90RSK1, RPS6KA1, MAPKAP kinase 1a, MAPK-activated protein kinase 1a, RSK-1, MAPKAPK1A, MAP kinase-activated protein kinase 1a, MAPKAPK-1a, S6K-alpha-1, 90 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase alpha-1, p90S6K
Background
Ribosomal protein S6 kinase alpha-1 (RPS6KA1) is a ~82 kDa protein. Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex.
1. Shimamura, A. et al. (2000) Current biology : CB 10, 127-35. PMID: 10679322
2. Wu, J. et al. (2002) The Journal of biological chemistry 277, 42669-79. PMID: 12213813
3. Hu, Y. et al. (2004) The Journal of biological chemistry 279, 29325-35. PMID: 15117958
4. Wingate, AD. et al. (2006) The Biochemical journal 393, 715-24. PMID: 16223362
5. Roux, PP. et al. (2007) The Journal of biological chemistry 282, 14056-64. PMID: 17360704
6. Carrière, A. et al. (2008) Current biology : CB 18, 1269-77. PMID: 18722121
7. Zhou, Y. et al. (2015) Nature communications 6, 7679. PMID: 26158630
中文
English
한국어
日本語
Español
Français
Русский
