Human RTCB Recombinant Protein (N-His) (HV728012)

Name: Human RTCB Recombinant Protein (N-His)
Brand: abinScience
SKU: HV728012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HV728012
Description	Recombinant Human RTCB Protein, N-His (HV728012) expressed in E. coli, spanning Met1-Gly505. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9Y3I0
Protein length	Met1-Gly505
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	57.37 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	C22orf28, RNA-splicing ligase RtcB homolog, RTCB, 3'-phosphate/5'-hydroxy nucleic acid ligase
Background	RNA-splicing ligase RTCB is a ~55 kDa protein. 3'-5' RNA ligase, catalytic subunit of the tRNA-splicing ligase complex (tRNA-LC), which is involved in the enzyme-dependent maturation of intron-containing pre-tRNAs. Functions downstream of the tRNA-splicing endonuclease that removes introns, ligating the two generated halves via phosphodiester bond formation. The ligation reaction, which requires guanosine triphosphate (GTP) and Mn(2+), proceeds through three metal-dependent steps. The first step requires ZBTB8OS/Archease and involves the guanylylation of RTCB at its active site histidine, forming a covalent GMP-histidine intermediate. Before the second step, RTCB also hydrolyzes the 2',3'-cyclic phosphate (cP) at the 3' end of the 5' tRNA exon, typically generated by the tRNA-splicing endonuclease, producing a 3' phosphate. 1. Loeff, L. et al. (2025) Nature structural & molecular biology 32, 1205-1212. PMID: 40069351 2. Popow, J. et al. (2014) Nature 511, 104-7. PMID: 24870230 3. Gerber, JL. et al. (2024) Nature communications 15, 2378. PMID: 38493148 4. Jurkin, J. et al. (2014) The EMBO journal 33, 2922-36. PMID: 25378478 5. Peschek, J. et al. (2015) EMBO reports 16, 1688-98. PMID: 26483401
Note	For research use only.

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SDS-PAGE for Recombinant Human RTCB Protein, N-His

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Human RTCB Recombinant Protein (N-His).pdf

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Human RTCB Recombinant Protein (N-His) (HV728012)

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