ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
EC:3.2.2.-, EC:3.2.2.6, HsTIR, KIAA0524, MyD88-5, NAD(+) hydrolase SARM1, NADP(+) hydrolase SARM1, NADase SARM1, SAM domain-containing protein 2, SAMD2, SARM, SARM1, Sterile alpha and Armadillo repeat protein, Sterile alpha and TIR motif-containing protein 1, Sterile alpha motif domain-containing protein 2, Tir-1 homolog, hSARM1
Background
NAD(+) hydrolase SARM1 is a ~79 kDa protein. NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism. Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site. Wallerian degeneration is triggered by NAD(+) depletion: in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting cytoskeletal degradation and axon destruction. Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules. Can activate neuronal cell death in response to stress.
1. Gerdts, J. et al. (2015) Science (New York, N.Y.) 348, 453-7. PMID: 25908823
2. Summers, DW. et al. (2016) Proceedings of the National Academy of Sciences of the United States of America 113, E6271-E6280. PMID: 27671644
3. Essuman, K. et al. (2017) Neuron 93, 1334-1343.e5. PMID: 28334607
4. Liberati, NT. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 6593-8. PMID: 15123841
5. Carty, M. et al. (2006) Nature immunology 7, 1074-81. PMID: 16964262
6. Peng, J. et al. (2010) European journal of immunology 40, 1738-47. PMID: 20306472
7. Murata, H. et al. (2018) The Journal of biological chemistry 293, 18933-18943. PMID: 30333228
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