Human SBF2/MTMR13 Recombinant Protein (N-GST & C-His) (HP597012)

Name: Human SBF2/MTMR13 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HP597012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP597012
Description	Recombinant Human SBF2/MTMR13 Protein, N-GST & C-His (HP597012) expressed in E. coli, spanning Gly775-Glu882. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q86WG5
Protein length	Gly775-Glu882
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	39.90 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	SBF2, SET-binding factor 2, Inactive phosphatidylinositol 3-phosphatase 13, CMT4B2, MTMR13, Myotubularin-related protein 13, KIAA1766
Background	Myotubularin-related protein 13 (SBF2/MTMR13) is a ~208 kDa protein. Guanine nucleotide exchange factor (GEF) which activates RAB21 and possibly RAB28. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. In response to starvation-induced autophagy, activates RAB21 which in turn binds to and regulates SNARE protein VAMP8 endolysosomal transport required for SNARE-mediated autophagosome-lysosome fusion. Acts as an adapter for the phosphatase MTMR2. Increases MTMR2 catalytic activity towards phosphatidylinositol 3,5-bisphosphate and to a lesser extent towards phosphatidylinositol 3-phosphate. 1. Yoshimura, S. et al. (2010) The Journal of cell biology 191, 367-81. PMID: 20937701 2. Jean, S. et al. (2015) EMBO reports 16, 297-311. PMID: 25648148
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human SBF2/MTMR13 Protein, N-GST & C-His

References

Datasheet

Human SBF2/MTMR13 Recombinant Protein (N-GST & C-His).pdf

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Formula

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Human SBF2/MTMR13 Recombinant Protein (N-GST & C-His) (HP597012)

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References

Datasheet

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