Human SEC11A Recombinant Protein (N-His) (HX979012)

Name: Human SEC11A Recombinant Protein (N-His)
Brand: abinScience
SKU: HX979012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX979012
Description	Recombinant Human SEC11A Protein, N-His (HX979012) expressed in E. coli, spanning Met1-Glu179. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P67812
Protein length	Met1-Glu179
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	22.79 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Microsomal signal peptidase 18 kDa subunit, Endopeptidase SP18, SPCS4A, Signal peptidase complex catalytic subunit SEC11A, SEC11A, SPase 18 kDa subunit, SEC11-like protein 1, SEC11 homolog A, SPC18, SEC11L1
Background	Signal peptidase complex catalytic subunit SEC11A is a ~20 kDa protein. Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids. 1. Liaci, AM. et al. (2021) Molecular cell 81, 3934-3948.e11. PMID: 34388369
Note	For research use only.

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SDS-PAGE for Recombinant Human SEC11A Protein, N-His

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Datasheet

Human SEC11A Recombinant Protein (N-His).pdf

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Human SEC11A Recombinant Protein (N-His) (HX979012)

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