Human SEC61A1 Recombinant Protein (N-GST & C-His) (HD113012)

Name: Human SEC61A1 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HD113012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD113012
Description	Recombinant Human SEC61A1 Protein, N-GST & C-His (HD113012) expressed in E. coli, spanning Ser376-Ala420. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P61619
Protein length	Ser376-Ala420
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.34 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Protein transport protein Sec61 subunit alpha isoform 1, SEC61A, SEC61A1, Sec61 alpha-1
Background	Protein transport protein Sec61 subunit alpha isoform 1 is a ~52 kDa protein. Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides. May cooperate with auxiliary protein SEC62, SEC63 and HSPA5/BiP to enable post-translational transport of small presecretory proteins. The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins: it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex. The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER. 1. Meacock, SL. et al. (2002) Molecular biology of the cell 13, 4114-29. PMID: 12475939 2. Lang, S. et al. (2012) Journal of cell science 125, 1958-69. PMID: 22375059 3. Schubert, D. et al. (2018) The Journal of allergy and clinical immunology 141, 1427-1438. PMID: 28782633 4. Haßdenteufel, S. et al. (2018) Cell reports 23, 1373-1386. PMID: 29719251 5. Chitwood, PJ. et al. (2020) Nature 584, 630-634. PMID: 32814900 6. McGilvray, PT. et al. (2020) eLife 9. PMID: 32820719 7. Sundaram, A. et al. (2022) Nature 611, 167-172. PMID: 36261522 8. Do, H. et al. (1996) Cell 85, 369-78. PMID: 8616892
Note	For research use only

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Human SEC61A1 Recombinant Protein (N-GST & C-His).pdf

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Human SEC61A1 Recombinant Protein (N-GST & C-His) (HD113012)

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