Human SIRT4 Recombinant Protein (N-His) (HV634012)

Name: Human SIRT4 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV634012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HV634012
Description	Recombinant Human SIRT4 Protein, N-His (HV634012) expressed in E. coli, spanning Ser28-Cys314. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9Y6E7
Protein length	Ser28-Cys314
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	34.48 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	NAD-dependent protein lipoamidase sirtuin-4, mitochondrial, SIR2-like protein 4, SIR2L4, NAD-dependent protein deacetylase sirtuin-4, NAD-dependent ADP-ribosyltransferase sirtuin-4, SIRT4, Regulatory protein SIR2 homolog 4
Background	NAD-dependent protein lipoamidase sirtuin-4, mitochondrial (SIRT4) is a ~35 kDa protein. Acts as a NAD-dependent protein lipoamidase, biotinylase, deacetylase and ADP-ribosyl transferase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. 1. Haigis, MC. et al. (2006) Cell 126, 941-54. PMID: 16959573 2. Ahuja, N. et al. (2007) The Journal of biological chemistry 282, 33583-33592. PMID: 17715127 3. Feldman, JL. et al. (2013) The Journal of biological chemistry 288, 31350-6. PMID: 24052263 4. Mathias, RA. et al. (2014) Cell 159, 1615-25. PMID: 25525879
Note	For research use only.

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SDS-PAGE for Human SIRT4 Recombinant Protein (N-His)

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Datasheet

Human SIRT4 Recombinant Protein (N-His).pdf

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Human SIRT4 Recombinant Protein (N-His) (HV634012)

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